C3an00266g 3216..3220
نویسندگان
چکیده
DNA detection based on peptide nucleic acid (PNA)–DNA hybridization is emerging as an important method in the area of DNA microarrays and biosensors because PNA shows remarkable hybridization properties. In this work, we provide a novel, simple, sensitive, and selective strategy based on a PNA– graphene oxide (GO) assembled biosensor for fluorescence turn-on detection of DNA, in which the new nanomaterial GO was used as a scaffold for PNA and a quencher for the fluorophore. The PNA–GO assembled biosensor is capable of distinguishing sequence specificity including complementary, onebase mismatched and non-complementary targets. Moreover, the results show that the biosensor is able to detect target DNA down to hundreds of picomolar. This sensing platform has been demonstrated to be highly sensitive and specific, and we expect that it will find great applications in the field of biomedicine and disease diagnostics.
منابع مشابه
Isochromophilones III-VI, inhibitors of acyl-CoA:cholesterol acyltransferase produced by Penicillium multicolor FO-3216.
New azaphilones named isochromophilones III-VI were isolated from the culture broth of Penicillium multicolor FO-3216 as inhibitors of acyl-CoA: cholesterol acyltransferase (ACAT). Their structures were elucidated by NMR and other spectroscopic analyses. The IC50 values of isochromophilones III, IV, V and VI for ACAT activity in an enzyme assay using rat liver microsomes were calculated to be 1...
متن کاملThink Locally, Act Locally: The Detection of Small, Medium-Sized, and Large Communities in Large Networks
Lucas G. S. Jeub, Prakash Balachandran, Mason A. Porter, 3 Peter J. Mucha, 5 and Michael W. Mahoney 7 Oxford Centre for Industrial and Applied Mathematics, Mathematical Institute, University of Oxford, OX2 6GG, UK Department of Mathematics & Statistics, Boston University, Boston, MA 02215, USA CABDyN Complexity Centre, University of Oxford, Oxford, OX1 1HP, UK Department of Applied Physical Sci...
متن کاملConstrained Control Problems of Discrete Processes
In undergoing this life, many people always try to do and get the best. New knowledge, experience, lesson, and everything that can improve the life will be done. However, many people sometimes feel confused to get those things. Feeling the limited of experience and sources to be better is one of the lacks to own. However, there is a very simple thing that can be done. This is what your teacher ...
متن کاملInsignificant human footprint on Sahel vegetation greenness
Disentangling the effects of climate and people on Sahel vegetation dynamics J. W. Seaquist, T. Hickler, L. Eklundh, J. Ardö, and B. W. Heumann Department of Physical Geography and Ecosystems Analysis, Geobiosphere Science Centre, Lund University, Sölvegatan 12, 223 62, Lund, Sweden Department of Geography, University of North Carolina at Chapel Hill, Saunders Hall, Campus Box 3220, Chapel Hill...
متن کامل